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As with the metabolic inhibition recordings, cells were perfused at a rate of 5 mL/min maintained at a temperature of 32C 2C using a Dagan HW-30 perfusion chamber (United States) and were stimulated to contract using electric field stimulation (EFS) at 1 Hz via a Digitimer DS stimulator (Welwyn Garden City, United Kingdom)

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References Hamman JJ, Kluess HA, Buckwalter JB, Clifford PS: Blood flow response to muscle contractions is more closely related to metabolic rate than contractile work

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This was demonstrated in a recent study whereby oral LC supplementation (250 mg for 12 weeks) among patients with PCOS improved total antioxidant capacity (TAC), decreased lipid peroxidation and enhanced general and mental health parameters [40]