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After transferring and blocking to nitrocellulose membranes, the blots were incubated overnight with primary antibodies against the target protein, at 4 C

doi: 10.1016/0305-0491(96)00022-3

Introduction The first reports about a plant glutathione S-transferase enzyme (GST, EC 2.5.1.18) appeared in 1970, when it was revealed that a GST catalyzed the detoxification of the herbicide atrazine by its conjugation to the endogenous tripeptide glutathione (GSH, -L-glutamyl-L-cysteinyl-glycine) in sorghum and maize plants (Frear and Swanson, 1970)

Strinic D, Belosic Halle Z, Luetic K, Nedic A, Petrovic I, Sucic M, Zivanovic Posilovic G, Balenovic D, Strbe S, Udovicic M, Drmic D, Stupnisek M, Lovric Bencic M, Seiwerth S, Sikiric P

The timeframe to see results from using the best glutathione brand can vary depending on various factors, including the individuals metabolism, dosage, and overall health

PMID 20603012