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RAW 264.7 murine macrophages were seeded at 1 10 5 cells mL 1 on 18 mm glass coverslips in a 12-well plate and allowed to attach overnight in Dulbeccos modified Eagle medium (DMEM, Corning) containing 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin at 37C and 5% CO 2

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