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Switching from later in the week to earlier Switching from a later day to an earlier day requires more planning because it means shortening the gap between doses

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For acylcarnitines, 30 L of the supernatant was added to 270 L of ice-cold methanol, vortexed, sonicated, and centrifuged (14,000 g, 4C, 15 minutes), and the supernatant was collected