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doi: 10.3389/fncir.2021.676891 88 DelattreVLa MendolaDMeystreJMarkramHMarkramK

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The fraction precipitating between 40 and 80% of the saturation in ammonium sulfate was subjected to a size-exclusion chromatography by loading the protein extract on an Ultrogel ACA44 (5 75 cm, Biosepra) column equilibrated with 30 mM Tris-HCl pH 8.0, 200 mM NaCl buffer

However, only EP300 and not CREBBP was significantly upregulated in RMC tumor cells of patients treated with ICT, suggesting that p300 is specifically engaged during RMC hyperprogression

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