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Add 1 mL of Reagent I (the proportion of tissue and reagents can be kept constant), fully grinding on ice (using liquid nitrogen will have a better grinding effect)

The antioxidant power of the intestine was compromised due to inhibition of key antioxidant enzymes and decreased reduced glutathione levels which led to impaired free radical quenching and metal-reducing ability

Stem Cell Transl Med

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We first identified decreased levels of antioxidant-related DEGs such as CAT , TXNRD3 , and GPX4 and total GSH levels that determine cellular redox potential were reduced by monobromobimane (mBBr) staining in the absence of glutamine, suggesting that glutamine metabolism inhibition only triggers oxidative stress (Fig