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10.1089/ars.2017.7342 197 ToosiS.OrlowS

Detection of S-glutathionylated FABP5 Cells were lysed in NP-40 lysis buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA] containing 20 mM N -ethylmaleimide (Sigma-Aldrich, E1271) and protease inhibitor cocktail (Roche, 04693132001)

Our ChIPqPCR analysis confirmed that STAT3 displayed high binding affinity with these two sites, which was dramatically increased upon amino acid treatment (Fig.7l)

There are six high-affinity binding proteins (insulin-like growth factor-binding protein [IGFBP]-1 to IGFBP-6) that are unrelated to the cell surface receptors [11]

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