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The solvent gradient (A/B/C) used for eluting estrogen metabolites is as follows: 8 min of isocratic at an initial composition of 16/68/16, 7 min of a concave gradient (curve number 9) to 18/64/18, 13 min of a concave gradient (curve number 8) to 20/59/21, 10 min of a convex gradient (curve number 2) to 22/57/21, 13 min of a concave gradient (curve number 8) to 58/21/21, followed by a 0.1-min step to 92/5/3 and a 8.9-min isocratic period at 92/5/3

Western blotting Cell pellets were lysed for 30 min in a lysis buffer (50 Mm Tris HCl pH 7.6, 150 mM NaCl, and 1% (v/v) NP-40) containing complete protease inhibitor cocktail (Sigma-Aldrich, 4693159001)

Mapping the zinc-transporting system in mammary cells: molecular analysis reveals a phenotype-dependent zinc-transporting network during lactation

Impaired neutrophil response and CD41 T helper cell 1 development in interleukin-6-deficient mice infected with Candida albicans

Our center currently administers 2 mg tPA intra-arterially to DCD graft recipients after reperfusion

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