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harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

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First, elucidating the cell-type-specific sensitivities and regulatory mechanisms of cuproptosis in major cardiac cells, including cardiomyocytes, ECs, and fibroblasts, is essential