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Within the research community, NNMT inhibition is studied in pre-clinical models for its relationship to the NAD+ salvage pathway, methyl-donor homeostasis, and adipocyte energy metabolism

233-235 In a randomized controlled trial in 84 subjects with coronary artery disease, 30 mg per day of crocin for eight weeks lowered levels of oxidized LDL and an inflammatory marker (monocyte chemoattractant protein-1, or MCP-1), and inhibited expression of genes related to atherosclerosis compared with placebo

The story is complex for two reasons

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We must obtain it through diet or supplementation, and its absorption involves a complex process that can easily be disrupted

Both protocols use a before-bed injection and run concurrently without modification to either schedule