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For the neuronal culture, hippocampi were dissected in HBSS, and digested for 1 h at 37 C in DMEM supplemented with 25 U/ml papain (Worthington Biomedical Corp., USA), 0.2 mg/ml cysteine (Merck, Germany), 1 mM CaCl 2 , and 0.5 mM EDTA

In the control group, an equivalent amount of saline was used

This preserves muscle mass and maintains metabolic rate during your cut

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We previously showed that FoxO1 governs the expression of lipid oxidative genes in white adipose cells via a redox-dependent manner 3,40

Each component contributes a distinct and complementary mechanism of action, making KLOW a subject of interest in multi-pathway research models