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Data processing The raw data were processed using Compound Discoverer 3.1 (CD3.1, ThermoFisher) for simple screening of parameters such as retention time and mass-to-charge ratio for each metabolite and then setting retention time deviation of 0.2 min and mass deviation of 5 ppm for peak alignment of different samples to make identification more accurate, followed by setting mass deviation of 5 ppm, signal intensity deviation of 30%, signal-to-noise ratio of 3, minimum signal intensity, summed ions, and other information for peak extraction, while quantifying the peak area, then integrating the target ions, predicting the molecular formula by molecular ion peaks and fragment ions and comparing with mzCloud ( mzVault and Masslist databases, removing the background ions with blank samples, and normalizing the original quantification results to obtain the relative peak areas

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The drugs molecular structure closely resembles that of acetylcholine and, therefore, activates the same receptors as acetylcholine

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3. Why is the Japan Version frequently referenced