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Microinjection and selection and confirmation of transgenic zebrafish To efficiently excise and integrate the DNA fragment surrounded by the Tol2 element transposon into the genome, we employed co-injection of in vitro-transcribed Tol2 transposase mRNA as described earlier 28

Despite their common use in clinical practice and short-term effectiveness for joint pain relief, recent studies indicate that intra-articular glucocorticoid injections are less effective than physical therapy in managing symptoms 1 year after administration (79)