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Cells were either lysed by sonication (4 20 s, 75% intensity with cooling breaks on ice) or H

All lines of evidence indicate that fast and slow TGR inhibitors bind different conformational states present in the TGR enzymatic cycle induced by both NADPH-dependent reduction and the concomitant destabilization of the polypeptide chain, as shown by TSA

10.1021/acs.orglett.9b01013 53 PiccirilloG.PepeA.BediniE.BochicchioB

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Bifunctional PEGylated exenatide-amylinomimetic hybrids to treat metabolic disorders: an example of long-acting dual hormonal therapeutics