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It has been reported that 91% of all signaling pathways and over 3,000 disease targets are related to AD [3]

A previous study identified genes necessary for MAP survival in dairy calves and permitted us to select a gene to knockout that is essential for MAP survival in vivo (MAP1643, IcL ) or one which is required for intracellular growth (MAP1531c, BacA ) ( Mouse and calf infection trials were employed to evaluate the immunogenicity of two vaccine candidates, confirm the attenuation in calf, and assess the species-specific essentiality of candidate genes

Testing sessions were conducted identically to training sessions