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Nowadays, in vitro -based biotechnological methods are applied for breeding with the aim of improving plant genotypes through rapid multiplication, micropropagation of disease-free plants, production of plant-derived metabolites, and gene transformation (Hesami et al., The genetic transformation of plantscan be achieved by direct and indirect methods (Niazian et al., Agrobacterium infection (Meyers et al., Agrobacterium method is a simple, efficient, and practical protocol for the transfer of foreign DNA and is the first prerequisite to produce genetically modified plants (Abbasi et al., Agrobacterium strain, Agrobacterium cell density, immersion time, type and concentration of antibiotics to kill Agrobacterium , type and concentration of the selected antibiotics, concentration of acetosyringone, duration of co-cultivation, pH and temperature of co-cultivation, and wounding treatments are the key factors that can affect Agrobacterium -mediated gene transformation and should be taken into account in all gene delivery studies (Liu et al., Agrobacterium -mediated gene delivery are Agrobacterium strain, Agrobacterium cell density, and antibiotic eliminating Agrobacterium , while the second group of influential factors is explant type and age along with immersion time and wounding treatment

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All of the above studies were limited to convenience samples and looked exclusively at cigarette smoking

Corrigan et al., 2003)