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For Pyridoxal-5-Phosphate (PLP, Sigma) supplementation-based growth kinetics, minimal medium (0.5 g/litre asparagine, 1 g/litre KH 2 PO 4 , 2.5 g/litre Na 2 HPO 4 , 50 mg/litre ferric ammonium citrate, 0.5 g/litre MgSO 4 .7H 2 O, 0.5 mg/litre CaCl 2 , and 0.1 mg/litre ZnSO 4 ) containing 0.1% glycerol (vol/vol) was used with or without 50 g/ml PLP (Sigma) supplementation