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The tissues were lysed in sonication buffer (20 mM HEPES pH7.5

Frequency of administration is decided by the treating physician

Ingredient sourcing is FDA-approved and logged per-drip

Many clinics schedule weekly IM visits for several weeks and reassess based on logs (nutrition, physical activity, sleep) and patient feedback

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Subsequently, this FDA approval led to BOTOX becoming the mainstay of non-surgical cosmetic procedures