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It demonstrates that the disruption of thiometabolism and disulfide stress play critical roles in CNS diseases, which are associated with the potential role of disulfidptosis

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siRNA for xCT and siPORT NeoFX transfect agent were procured from Ambion, Texas, USA

Seeds were surface sterilized with bleach (3% sodium hypochlorite) for 8 min before being plated on half-strength sucrose-free Murashige and Skoog (1/2 MS0) media supplemented with Gamborgs vitamins (Caisson Laboratories, North Logan, UT), 0.5 mM MES (pH 5.7), and 0.8% (w/v) agar (Caisson Laboratories, North Logan, UT)