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These results confirmed that the enzymatic reaction catalysed by Pt GSTF8 terminated the hydration equilibrium between flavan-3,3,4-triol ( 4 ) and flavan-3-on-4-ol ( 7 ), leading us to propose a GST-dependent dehydration of flavan-3,3,4-triol ( 4 ) or a tautomerization of flavan-3-on-4-ol ( 7 ) to form flav-2-en-3,4-diol ( 8 -B4), an equilibrium form of anthocyanins ( 1 ) in solution (Fig