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The oligo was dried using a SpeedVac, resuspended in water, applied to a MonoQ 5/50 GL column in buffer A (10 mM TRIS-HCL pH 7.5, 100 mM NaCl) and eluted in a gradient of 3% buffer B (10 mM TRIS-HCL pH 7.5, 800 mM NaCl) per CV at 4 C

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