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Cell 2014;159(7):1615-25

For example, in a rat model of middle cerebral artery occlusion (MCAO), T

Cells were permeabilized in 0.2% (v/v) Triton X-100 for 5 min, blocked in 1% bovine serum albumin and 2% donkey serum for 30 min., incubated with primary antibodies for 1 h, washed with PBS and finally incubated with FITC- or Cy3- conjugated secondary antibodies (Jackson ImmunoResearch)

& Duffull, S

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