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Defective myofibroblast formation from mesenchymal stem cells in the aging murine heart rescue by activation of the AMPK pathway

Cells were cultured in high-glucose DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin, and maintained in a humidified incubator at 37 C with 5% CO

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Running the freezer alone costs about 1,500 shekels a month ($488), while electricity bills reach 1,3001,400 ($423-$456) shekels every 10 days, with power costing about 35 shekels ($11.39) per kilowatt