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Cyclophosphamide (Cy) is an alkylating agent with antineoplastic and immunosuppressive activity, whose bioactivation requires the activity of polymorphic enzymes in the liver to produce phosphoramide mustard, which is a DNA alkylating agent

Gut bacteria ferment it in the large intestine, producing gas as a byproduct

Moreover, glutathione was found to alleviate hypoxia-induced endoplasmic reticulum stress (ERS) in gills, a response potentially linked to the suppression of PERK, IRE1, and ATF6 signaling pathways

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Our work expands our understanding of the downstream molecular events that are associated with antibiotic lethality, revealing cytoplasmic condensation as a phenotypic feature of antibiotic-induced bacterial cell death

Considering the relationships between amount of drug, A , volume of distribution, V , and drug concentration, C , of Eq