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Apoptosis-inducing factor (AIF) and its family member protein, amid, are rotenone-sensitive nadh:ubiquinone oxidoreductases (NDH-2)

These insights will enhance our understanding of the molecular mechanisms underlying orthopedic diseases and highlight potential molecular targets for developing new therapeutic strategies

All experimental procedures were performed in accordance with the International Code of Ethics, ARRIVE guidelines, and the National Research Council's Guide for the Care and Use of Laboratory Animals

After 2 days, the cells were first treated with the indicated compounds for 4 h and then with 30 or 100 nM MZ1 for an additional 2 h