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Cells were fixed by vortexing with simultaneous addition of Complete Cell Fixation Solution (Fixation Reagent1, ice-cold 100% methanol, Stanlab, Poland) with DEPC, followed by 15 min incubation on ice and then washed with WRS Buffer (Scale Biosciences) and resuspended in WRS Buffer

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The generation of OH provokes the oxidation of PUFAs and thereby initiates the cascade leading to ferroptosis

El hecho de que el nivel de GSH est comprometido en individuos infectados con HIV y que esto juegue un rol muy importante en la patognesis de la enfermedad est bien documentado aunque todava se desconozcan los mecanismos responsables de la deficiencia de GSH

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In addition to glycolysis, astrocytes store energy by synthesizing glycogen via Glc-6-P