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Then, cells were washed in PBS-BSA 0.5% with 0.02% sodium azide, centrifuged at 1,500 rpm for 5 min at 4 C, counted again, and separated as 2 10 5 cells per tube

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Abramovitz M, Homma H, Ishigaki S, Tansey F, Cammer W, Listowsky I: Characterization and localization of glutathione-S-transferases in rat brain and binding of hormones, neurotransmitters, and drugs

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Immunity 42 , 4154 (2015)