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For the colony formation assay, cells in exponential growth with viability above 95% were digested, dispersed into single-cell suspensions, and counted

ZZW-115 prevents the complex formation between NUPR1 and importin, which inhibits the nuclear translocation of NUPR1 and thus NUPR1-induced gene transcription 39

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DSIP was well tolerated in all published clinical trials, with a tolerability rate of 97%